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s366 p sting (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc s366 p sting
$( A ) Western blot analysis of subcellular fractions from HDF treated for 6 days with doxycycline (Dox) to induce progerin expression, for 24 h with hydroxyurea (HU), or transfected with single-stranded DNA (ssDNA). Fraction markers include β-tubulin (cytoplasm), SEC61 (membrane), Lamin A (nucleus), and Histone H3 (chromatin). Note the increased presence of STING at nucleus and chromatin upon replication stress. Membranes imaged with prolonged exposure (high exposure) show a marked increase in signal intensity. ( B ) Immunofluorescence (IF) with STING antibody in HDF treated with vehicle, ssDNA, HU, Doxy (progerin), or dsDNA. ( C ) Quantification of cGAMP levels measured by ELISA in HDF treated as indicated. ( D ) IF with STING antibody and quantification of percentage of cells showing STING localization to the perinuclear compartment (PNC) upon different treatments. ( E ) IF with antibody recognizing phosphorylated STING on Ser366 and quantification of percentage of cells positive for <t>S366</t> p-STING. ( F ) Immunoblot analysis of STING, GFP-progerin, and markers of activation of the canonical cGAS-STING pathway ( S366 p-STING, S386 p-IRF3, and S172 p-TBK1) following treatments. ( G ) Immunoblot analysis of STING pathway components and ISG proteins (STAT1, S727 p-STAT1, RIG-I, and ISG15) after indicated treatments.$
S366 P Sting, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s366+p+sting/bio_rxiv__64898__2026__03__28__714577-203-26-29?v=Cell+Signaling+Technology+Inc
Average 86 stars, based on 1 article reviews

s366 p sting - by Bioz Stars, 2026-07

86/100 stars

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1) Product Images from "STING causes replication stress and nascent DNA degradation via SAMHD1"

Article Title: STING causes replication stress and nascent DNA degradation via SAMHD1

Journal: bioRxiv

doi: 10.64898/2026.03.28.714577

$( A ) Western blot analysis of subcellular fractions from HDF treated for 6 days with doxycycline (Dox) to induce progerin expression, for 24 h with hydroxyurea (HU), or transfected with single-stranded DNA (ssDNA). Fraction markers include β-tubulin (cytoplasm), SEC61 (membrane), Lamin A (nucleus), and Histone H3 (chromatin). Note the increased presence of STING at nucleus and chromatin upon replication stress. Membranes imaged with prolonged exposure (high exposure) show a marked increase in signal intensity. ( B ) Immunofluorescence (IF) with STING antibody in HDF treated with vehicle, ssDNA, HU, Doxy (progerin), or dsDNA. ( C ) Quantification of cGAMP levels measured by ELISA in HDF treated as indicated. ( D ) IF with STING antibody and quantification of percentage of cells showing STING localization to the perinuclear compartment (PNC) upon different treatments. ( E ) IF with antibody recognizing phosphorylated STING on Ser366 and quantification of percentage of cells positive for S366 p-STING. ( F ) Immunoblot analysis of STING, GFP-progerin, and markers of activation of the canonical cGAS-STING pathway ( S366 p-STING, S386 p-IRF3, and S172 p-TBK1) following treatments. ( G ) Immunoblot analysis of STING pathway components and ISG proteins (STAT1, S727 p-STAT1, RIG-I, and ISG15) after indicated treatments.$
Figure Legend Snippet: ( A ) Western blot analysis of subcellular fractions from HDF treated for 6 days with doxycycline (Dox) to induce progerin expression, for 24 h with hydroxyurea (HU), or transfected with single-stranded DNA (ssDNA). Fraction markers include β-tubulin (cytoplasm), SEC61 (membrane), Lamin A (nucleus), and Histone H3 (chromatin). Note the increased presence of STING at nucleus and chromatin upon replication stress. Membranes imaged with prolonged exposure (high exposure) show a marked increase in signal intensity. ( B ) Immunofluorescence (IF) with STING antibody in HDF treated with vehicle, ssDNA, HU, Doxy (progerin), or dsDNA. ( C ) Quantification of cGAMP levels measured by ELISA in HDF treated as indicated. ( D ) IF with STING antibody and quantification of percentage of cells showing STING localization to the perinuclear compartment (PNC) upon different treatments. ( E ) IF with antibody recognizing phosphorylated STING on Ser366 and quantification of percentage of cells positive for S366 p-STING. ( F ) Immunoblot analysis of STING, GFP-progerin, and markers of activation of the canonical cGAS-STING pathway ( S366 p-STING, S386 p-IRF3, and S172 p-TBK1) following treatments. ( G ) Immunoblot analysis of STING pathway components and ISG proteins (STAT1, S727 p-STAT1, RIG-I, and ISG15) after indicated treatments.

Techniques Used: Western Blot, Expressing, Transfection, Membrane, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Activation Assay

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Journal: bioRxiv

Article Title: STING causes replication stress and nascent DNA degradation via SAMHD1

doi: 10.64898/2026.03.28.714577

Figure Lengend Snippet: ( A ) Western blot analysis of subcellular fractions from HDF treated for 6 days with doxycycline (Dox) to induce progerin expression, for 24 h with hydroxyurea (HU), or transfected with single-stranded DNA (ssDNA). Fraction markers include β-tubulin (cytoplasm), SEC61 (membrane), Lamin A (nucleus), and Histone H3 (chromatin). Note the increased presence of STING at nucleus and chromatin upon replication stress. Membranes imaged with prolonged exposure (high exposure) show a marked increase in signal intensity. ( B ) Immunofluorescence (IF) with STING antibody in HDF treated with vehicle, ssDNA, HU, Doxy (progerin), or dsDNA. ( C ) Quantification of cGAMP levels measured by ELISA in HDF treated as indicated. ( D ) IF with STING antibody and quantification of percentage of cells showing STING localization to the perinuclear compartment (PNC) upon different treatments. ( E ) IF with antibody recognizing phosphorylated STING on Ser366 and quantification of percentage of cells positive for S366 p-STING. ( F ) Immunoblot analysis of STING, GFP-progerin, and markers of activation of the canonical cGAS-STING pathway ( S366 p-STING, S386 p-IRF3, and S172 p-TBK1) following treatments. ( G ) Immunoblot analysis of STING pathway components and ISG proteins (STAT1, S727 p-STAT1, RIG-I, and ISG15) after indicated treatments.

Article Snippet: Primary antibodies used were β-tubulin (1:5000- Origene-AP31823PU-N), GAPDH (1:1000- Cell Signaling-2118), Lamina A (1:3000- Abcam-1791), Progerin (1:1000 Santa Cruz-81511), ISG15 (1:1000 Santa Cruz-166755), STING (1:1000-Cell Signaling-13647), S366 p-STING (1:1000- Cell Signaling- 50907), RIG-I (1:1000-Cell Signaling-3743S), S33-p-RPA (1:1000 Bethyl- PLA0070), SAMHD1 (1:1000- Cell Singaling-49158), λH2AX (1:1000- Cell Signaling- 2577).

Techniques: Western Blot, Expressing, Transfection, Membrane, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Activation Assay